Effect of hemicellulose and lignin on enzymatic hydrolysis of cellulose from brewer's spent grain

  title={Effect of hemicellulose and lignin on enzymatic hydrolysis of cellulose from brewer's spent grain},
  author={Solange I. Mussatto and Marcela Fernandes and Adriane Maria Ferreira Milagres and In{\^e}s Conceiç{\~a}o Roberto},
  journal={Enzyme and Microbial Technology},
Effect of Xylan and Lignin Removal by Hydrothermal Pretreatment on Enzymatic Conversion of Sugarcane Bagasse Cellulose for Second Generation Ethanol Production
This work was aimed to evaluate the effect of the removal of hemicellulose and lignin, by hydrothermal pretreatment, carried out at four different temperatures, namely 180, 185, 190 and 195 °C, for
Impact of Hemicellulose on Cellulose Simultaneous Saccharification and Fermentation
Hemicellulose is generally considered a negative factor for enzymatic cellulose hydrolysis, but it was proved to be a positive one for the enzymatic action of delignified pulp in this study. Alkaline
Hydrolysis of Brewers' Spent Grain by Carbohydrate Degrading Enzymes
The unhydrolysed fraction was still found to contain over 40% of carbohydrates after enzymatic treatment despite the extensive enzyme dosages used, indicating that the majority of the BSG biomass is rather recalcitrant towards the cellulose-hemicellulase enzyme mixtures applied in this study.
The Effect of Xylan Removal on the High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse
Although high-solid enzymatic hydrolysis has emerged as an efficient strategy of sugar production from lignocellulosic materials, this process requires several steps to increase its efficiency.
The Effects of Surfactant Pretreatment and Xylooligomers on Enzymatic Hydrolysis of Cellulose and Pretreated Biomass
Investigating the effects of hemicelluloses on enzymatic hydrolysis and developing possible strategies to overcome their negative impacts suggested that xylooligomers could competitively adsorb on cellulase, thereby reducing enzyme accessibility to cellulose and celloolsigomers.
Characteristics of plant biomass and their effect on enzymatic saccharification
Enzymatic hydrolysis of a large number of not-treated and pretreated plant materials of various origins containing different amounts of cellulose, hemicelluloses and lignin has been studied. To
Characterization of commercial cellulases and their use in the saccharification of a sugarcane bagasse sample pretreated with dilute sulfuric acid
This study aimed to correlate the efficiency of enzymatic hydrolysis of the cellulose contained in a sugarcane bagasse sample pretreated with dilute H2SO4 with the levels of independent variables
Evaluation of cellulosic and hemicellulosic hydrolysate fermentability from sugarcane bagasse hybrids with different compositions
Seven sugarcane hybrids with varying initial level of cell wall components were evaluated as substrates to ethanol production from cellulosic and hemicellulosic fractions by Pichia stipitis NRRL
An approach to cellulase recovery from enzymatic hydrolysis of pretreated sugarcane bagasse with high lignin content
It was concluded that it is possible to recycle cellulases from an enzymatic medium for use in subsequent hydrolysis processes.
Improvement of cassava stem hydrolysis by two-stage chemical pretreatment for high yield cellulosic ethanol production
We used sodium chlorite followed by sodium hydroxide as a two-stage pretreatment of cassava stem for removal of lignin and hemicellulose to obtain a substrate with high cellulose content prior to


Effects of hemicellulose and lignin on enzymatic hydrolysis of cellulose from dairy manure
Quantitatively, fed-batch enzymatic hydrolysis of fiber without lignin and hemicellulose had a high glucose yield of 52% with respect to the glucose concentration of 17 g/L at a total enzyme loading of 1300 FPU/L and reaction time of 160 h, which was better than corresponding batch enzyme loading.
Acid hydrolysis and fermentation of brewer's spent grain to produce xylitol
The hemicellulosic fraction of brewer's spent grain (BSG) was hydrolysed with diluted acid under different conditions of liquid/solid ratio (8–12 g g−1), sulfuric acid concentration (100–140 mg g−1
Chemical characterization and liberation of pentose sugars from brewer's spent grain
The chemical composition of brewer's spent grain (BSG), generated from a process using 100% malted barley, was investigated. BSG is mainly composed of (g kg−1) hemicellulose (284), lignin (278),
Adsorption of cellulase from Trichoderma reesei on cellulose and lignacious residue in wood pretreated by dilute sulfuric acid with explosive decompression
The data show that the pretreatment at a higher temperature results in more enzyme adsorption on the cellulose fraction and less on the lignacious residue fraction, and the relationship between the hydrolysis rate and the amount of enzymes adsorbed is discussed.
Factors affecting cellulose hydrolysis and the potential of enzyme recycle to enhance the efficiency of an integrated wood to ethanol process
A comparison of the front end (pretreatment, fractionation, and hydrolysis) of a softwood/hardwood to ethanol process indicated that the lignin associated with the softwood‐derived cellulose stream limited the number of times the cellulose containing residue could be recycled.
Strategies to enhance the enzymatic hydrolysis of pretreated softwood with high residual lignin content
Pretreatment of Douglas-fir by steam explosion produces a substrate containing approx 43% lignin. Two strategies were investigated for reducing the effect of this residual lignin on enzymatic