Effect of growth hormone and glucose on rat islet cells replication using 5-bromo-2-deoxyuridine incorporation.

Abstract

Islet cell proliferation was quantified by labelling fetal and adult rat islets in vitro with 5-bromo-2-deoxyuridine (BrdU), a thymidine analog that cannot be reutilized by the cell. After incubation with BrdU, cells were dispersed, centrifuged onto slides and DNA labeled with BrdU detected by a fluorescent anti BrdU antibody. The fluorescence was always… (More)

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