Effect of glycosylation inhibitors on the production and properties of human leukocyte interferon.

@article{Stewart1979EffectOG,
  title={Effect of glycosylation inhibitors on the production and properties of human leukocyte interferon.},
  author={W. E. Stewart and Marzenna Wiranowska-Stewart and Vesa Koistinen and Kari Cantell},
  journal={Virology},
  year={1979},
  volume={97 2},
  pages={
          473-6
        }
}
Abstract Human leukocyte “buffy coat” suspensions were induced to produce interferon by stimulation with Sendai virus and were incubated during the interferon production period in medium containing various concentrations of glycosylation inhibitors, either d -glucosamine or 2-deoxy-D-glucose. At 80 mM d -glucosamine or at 8 mM 2-deoxy- d -glucose, interferon production was inhibited by approximately 95%; however, at 20 and 2 mM, respectively, these inhibitors allowed interferon production at… Expand
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TLDR
Interferon synthesized in the presence of partially inhibitory concentrations of 2-deoxy-D-glucose or D- glucosamine were found to differ from interferons made in control cultures in some physical properties. Expand
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TLDR
The results are compatible with the idea that the two inhibitors suppress the production of biologically active interferon by virtue of their interfering with the proper glycosylation of the molecule. Expand
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TLDR
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TLDR
Human lymphoblastoid interferon was produced on an 800-liter scale by induction of Namalva cells with Newcastle disease virus, strain B1 and could be completely (85 to 113%) recovered from the gels by elution into a buffer containing sodium dodecyl sulfate. Expand
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TLDR
The data suggest that certain interferons can be modified to attain a conformation which, though inactive in homologous cells, is fully able to induce antiviral activity in heterologous Cells. Expand
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TLDR
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TLDR
Human leukocyte and tritium-labeled fibroblast interferons, prepared by induction with Sendai virus and with double-stranded polyinosinic acid respectively, have been studied in relation to the carbohydrate moieties attached to them to reduce the heterogeneous character of interferon. Expand
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TLDR
It is indicated that the two components, human leukocyte interferon and rabbit anti-leukocyteinterferon, posses at most only minor structural and antigenic dissimilarities. Expand
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TLDR
It is reported that human leukocyte interferon preparations do, indeed, contain two molecular populations that can be distinguished physically, chemically, and biologically. Expand
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