Effect of gelation conditions on the gel structure and resolving power of agarose-based DNA sequencing gels.

@article{Kusukawa1999EffectOG,
  title={Effect of gelation conditions on the gel structure and resolving power of agarose-based DNA sequencing gels.},
  author={Noriko Kusukawa and Mikhail Ostrovsky and M. M. Garner},
  journal={Electrophoresis},
  year={1999},
  volume={20 7},
  pages={1455-61}
}
The effect of gelation conditions on the structure and resolving properties of agarose-based DNA sequencing gels were studied. Gels that were formed by rapid cooling gave separation patterns with sharp, well-defined bands, and resolved base repeats that were not separated on gels which were allowed to cool slowly. Analyses by electron microscopy indicated that the rapidly cooled gels have a homogeneous pore structure with a smaller average pore size than gels that were cooled slowly. The two… CONTINUE READING