Received: 10 January, 2016 Accepted: 17 March, 2016 Published: 20 June, 2016 Gamma ray was applied to reduce mycotoxins, i.e. ochratoxin A (OTA) and aflatoxins B1, B2, G1 and G2 (AFB1, AFB2, AFG1 and AFG2) in black and white pepper. The aim of the this study was to evaluate the efficacy of gamma radiation for decontaminating ochratoxin) and aflatoxins residues in artificially contaminated black and white pepper samples. The moisture content of the pepper samples was set at 10% or 16%, and the applied gamma dose ranged from 5 to 20 kGy. Mycotoxin levels were determined by high-performance liquid chromatography (HPLC) after immune affinity column (IAC) chromatography. Both the gamma irradiation dose and moisture content showed significant effects (p<0.05) on mycotoxin reduction. The maximum toxin reductions, found at16%moisture content and 20 kGy, were 57.2%, 53.4%, 40.4%, 48.6% and 44.2% for OTA, AFB1, AFB2, AFG1and AFG2, respectively. © 2016PSCI Publisher All rights reserved.