Effect of furosemide on prostaglandin synthesis by human nasal and bronchial epithelial cells in culture.

@article{LEVASSEURACKER1994EffectOF,
  title={Effect of furosemide on prostaglandin synthesis by human nasal and bronchial epithelial cells in culture.},
  author={G. LEVASSEUR‐ACKER and M. Molimard and J. Regnard and E. Naline and C. Fr{\`e}che and A. Lockhart},
  journal={American journal of respiratory cell and molecular biology},
  year={1994},
  volume={10 4},
  pages={
          378-83
        }
}
Inhaled furosemide protects asthmatic subjects against bronchial obstruction caused by indirect provocants. We have attempted to correlate the protective effect of furosemide with its ability to alter prostaglandin (PG) synthesis by the airway epithelium. Human epithelial cells from nasal polyps and bronchi were cultured in DME-Ham's F12 medium with 10% fetal calf serum. Confluent cells (days 6 through 8) were incubated for 30 min in fresh medium, and the PGs in the supernatant were measured by… Expand
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