Effect of free fatty acids on the binding kinetics at the benzodiazepine binding site of glycated human serum albumin.

@article{Keita1993EffectOF,
  title={Effect of free fatty acids on the binding kinetics at the benzodiazepine binding site of glycated human serum albumin.},
  author={Y Keita and Wolfgang Kratzer and Wolf Woerner and Norbert Rietbrock},
  journal={International journal of clinical pharmacology, therapy, and toxicology},
  year={1993},
  volume={31 7},
  pages={337-42}
}
The effect of free fatty acids (FFA) and non-enzymatic glycation on the binding kinetics of dansylsarcosine (DS) to human serum albumin (HSA) was studied using the stopped-flow technique. The influence of FFA on the binding parameters of 25% glycated HSA depended on the type of fatty acid. The addition of stearic, oleic and linoleic acids in a concentration of 0.3 mmol/l showed no inhibitory effects on the association rate constant (k2) value for DS binding to 25% glycated HSA (k2 without FFA… CONTINUE READING