Effect of formaldehyde on the efficiency of hybridization of DNA immobilized on nitrocellulose filters.

@article{Ivanov1992EffectOF,
  title={Effect of formaldehyde on the efficiency of hybridization of DNA immobilized on nitrocellulose filters.},
  author={Eugenyi Ivanov and M G Abouhaidar},
  journal={Analytical biochemistry},
  year={1992},
  volume={206 2},
  pages={
          414-8
        }
}
We report in this study that under certain conditions formaldehyde interacts with DNA and makes it more efficient for hybridization on nitrocellulose filters. Hybridization signals of formaldehyde-treated DNA are stronger (up to 10 fold) as compared with that of the heat- or alkali-denatured DNA. Various parameters of the DNA-formaldehyde reaction are optimized as follows: (a) 6 x SSC, 10% formaldehyde, 60 degrees C, 20-30 min, reaction volume 10-200 microliters or (b) 6 x SSC, 5% formaldehyde… CONTINUE READING
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