Effect of fluid flow on smooth muscle cells in a 3-dimensional collagen gel model.

@article{Wang2000EffectOF,
  title={Effect of fluid flow on smooth muscle cells in a 3-dimensional collagen gel model.},
  author={Shiyu Wang and John M. Tarbell},
  journal={Arteriosclerosis, thrombosis, and vascular biology},
  year={2000},
  volume={20 10},
  pages={2220-5}
}
A 3D collagen gel model was developed to simulate interstitial fluid flow and to assess the importance of this flow on the biochemical production rates of vascular smooth muscle cells (SMCs). Rat aortic SMCs were suspended in type I collagen, and the gel was supported by nylon fibers that allowed a 9-cm length of the SMC-gel model to withstand 90 cm H(2)O differential pressure over a 6-hour period without significant compaction. Up to 1 dyne/cm(2) shear stress on the suspended SMCs could be… CONTINUE READING