Effect of extraction method on activities of polysaccharide-depolymerase enzymes in the microbial population from the solid phase in the rumen.

@article{Nozire1994EffectOE,
  title={Effect of extraction method on activities of polysaccharide-depolymerase enzymes in the microbial population from the solid phase in the rumen.},
  author={Pierre Nozi{\`e}re and Brigitte Michalet-Doreau},
  journal={Reproduction, nutrition, development},
  year={1994},
  volume={34 4},
  pages={281-8}
}
Samples of rumen digesta were collected from 2 cows fed with a 100:0 or 40:60 forage/concentrate diet 1 h before and 2 h after feeding. Enzymes from particle-associated microorganisms were extracted by 5 different methods, including physical (freezing, grinding and sonication) or chemical and enzymatic (autolysis in a buffer, lysozyme and CCl4) treatments. Hydrolytic enzyme activities were measured on 3 polysaccharidic substrates: xylan, carboxymethylcellulose and Avicel cellulose… CONTINUE READING