Effect of exposure duration of ovaries and oocytes at ambient temperature on parthenogenetic development of porcine follicular oocytes.

@article{Kim2006EffectOE,
  title={Effect of exposure duration of ovaries and oocytes at ambient temperature on parthenogenetic development of porcine follicular oocytes.},
  author={Hyun-Jong Kim and Sun-Ho Choi and Dong-Soo Son and S Cho and Chang-yong Choe and Young-Keun Kim and Man-Hye Han and Il-Sun Ryu and In-Cheul Kim and Il-Hwa Kim and Kyung-Soon Im and Takashi Nagai},
  journal={The Journal of reproduction and development},
  year={2006},
  volume={52 5},
  pages={633-8}
}
This study was conducted to evaluate the effects of exposing porcine ovaries to 30-33 C during transportation for 4 h and subsequently room temperature (25 C) for 6 h of storage on in vitro maturation (IVM) and subsequent parthenogenetic development of oocytes collected from the ovaries. After IVM, oocytes having a tight oopalsm membrane and no signs of degeneration were exposed to Dulbecco's phosphate-buffered saline (DPBS) with 7% ethanol (v/v) for 7 min to induce parthenogenetic activation… CONTINUE READING