Effect of estrogen on gene expression in the chick oviduct. In vitro transcription of the ovalbumin gene in chromatin.

@article{Harris1976EffectOE,
  title={Effect of estrogen on gene expression in the chick oviduct. In vitro transcription of the ovalbumin gene in chromatin.},
  author={Stephen E. Harris and Robert J. Schwartz and M. J. Tsai and Bert W O'malley and A. K. Roy},
  journal={The Journal of biological chemistry},
  year={1976},
  volume={251 2},
  pages={524-9}
}
RNA was transcribed from chromatin isolated from chick oviduct and spleen by using RNA polymerase from Escherichia coli. RNA was also transcribed from whole chick DNA using E. coli RNA polymerase. DNA complementary to ovalbumin messenger RNA (cDNAov) was then used as a hybridization probe to estimate the concentration of ovalbumin messenger RNA sequences (mRNAov) in these in vitro transcripts. Although chromatin from unstimulated chick oviduct was capable of substantial RNA synthesis, no… CONTINUE READING
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