Effect of dynamic 3-D culture on proliferation, distribution, and osteogenic differentiation of human mesenchymal stem cells.

@article{Stiehler2009EffectOD,
  title={Effect of dynamic 3-D culture on proliferation, distribution, and osteogenic differentiation of human mesenchymal stem cells.},
  author={Maik Stiehler and Cody Eric B{\"u}nger and Anette Baatrup and Martin Lind and Moustapha Kassem and Tina Mygind},
  journal={Journal of biomedical materials research. Part A},
  year={2009},
  volume={89 1},
  pages={96-107}
}
Ex vivo engineering of autologous bone tissue as an alternative to bone grafting is a major clinical need. In the present study, we evaluated the effect of 3-D dynamic spinner flask culture on the proliferation, distribution, and differentiation of human mesenchymal stem cells (MSCs). Immortalized human MSCs were cultured on porous 75:25 PLGA scaffolds for up to 3 weeks. Dynamically cultured cell/scaffold constructs demonstrated a 20% increase in DNA content (21 days), enhanced ALP specific… CONTINUE READING

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