Effect of different culture conditions on establishment of embryonic stem cells from BALB/cAJ and NZB/BINJ mice.

@article{Iijima2010EffectOD,
  title={Effect of different culture conditions on establishment of embryonic stem cells from BALB/cAJ and NZB/BINJ mice.},
  author={Saori Iijima and Yoko Tanimoto and Seiya Mizuno and Yoko Daitoku and Satoshi Kunita and Fumihiro Sugiyama and Ken-ichi Yagami},
  journal={Cellular reprogramming},
  year={2010},
  volume={12 6},
  pages={679-88}
}
As the phenotype of a given single-gene mutation in mice is modulated by the genetic background of the inbred strain, embryonic stem (ES) cells derived from various inbred mouse strains are required to produce gene-targeted mice without the need for backcrossing and for detailed analysis of gene function in vivo. Here, we performed a comparative investigation of the effects of three culture conditions, LIF + KSR/ES medium described previously, High LIF + KSR/ES medium and iSTEM + LIF medium… CONTINUE READING