Corpus ID: 82012113

Effect of dietary fat manipulation and calorie restriction on collagen in ageing skeletal muscle.

@article{Kuriakose2011EffectOD,
  title={Effect of dietary fat manipulation and calorie restriction on collagen in ageing skeletal muscle.},
  author={M. Kuriakose and V. Leela and A. Thangavel and G. Ramesh},
  journal={Journal of Indian Veterinary Association, Kerala},
  year={2011},
  volume={9},
  pages={32-34}
}
The rats were sacrificed at the age of 24 months. The gastrocnemius muscle was collected for histological and histochemical analysis of collagen as per the procedure of Neuman and Logan (1950). Collagen was estimated measuring hydroxyl proline which is the functional component of collagen. Histology of collagen was carried out from paraffin embedded 5-6 μm sections of gastrocnemius muscle using Masson ́s trichrome staining technique and histochemistry by per-iodic acid-Schiff method (PAS) (Luna… Expand

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