Effect of detergents, trypsin and unsaturated fatty acids on latent loquat fruit polyphenol oxidase: basis for the enzyme's activity regulation.

@article{SellsMarchart2007EffectOD,
  title={Effect of detergents, trypsin and unsaturated fatty acids on latent loquat fruit polyphenol oxidase: basis for the enzyme's activity regulation.},
  author={Susana Sell{\'e}s-Marchart and Juan Casado-Vela and Roque Bru-Mart{\'i}nez},
  journal={Archives of biochemistry and biophysics},
  year={2007},
  volume={464 2},
  pages={
          295-305
        }
}
The effects of detergents, trypsin and fatty acids on structural and functional properties of a pure loquat fruit latent polyphenol oxidase have been studied in relation to its regulation. Anionic detergents activated PPO at pH 6.0 below critical micelle concentration (cmc), but inhibited at pH 4.5 well above cmc. This behavior is due to a detergent-induced pH profile alkaline shift, accompanied by changes of intrinsic fluorescence of the protein. Gel filtration experiments demonstrate the… CONTINUE READING