Effect of detector wavelength on the determination of the molecular weight of humic substances by high-pressure size exclusion chromatography.

@article{OLoughlin2001EffectOD,
  title={Effect of detector wavelength on the determination of the molecular weight of humic substances by high-pressure size exclusion chromatography.},
  author={Emmett O'Loughlin and Yuk Ping Chin},
  journal={Water research},
  year={2001},
  volume={35 1},
  pages={333-8}
}
High-pressure size exclusion chromatography (HPSEC) has proven to be an effective method for determining the molecular weights (MW) of humic substances (HS) from a variety of aquatic and terrestrial environments. HPSEC systems often use a variable wavelength UV-vis detector, which detects the analytes based upon their chromophoric composition. HS contain a range of moieties with chromophores having unique molar absorptivities (for a given wavelength), and the calculated MW may be dependent upon… CONTINUE READING

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