Effect of demecolcine and nocodazole on the efficiency of chemically assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes.

@article{Kawakami2003EffectOD,
  title={Effect of demecolcine and nocodazole on the efficiency of chemically assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes.},
  author={Masayoshi Kawakami and Tetsuya Tani and Akiko Yabuuchi and Tatsuya Kobayashi and Hiroshi Murakami and Tatsuya Fujimura and Yoshiya Kato and Yukio Tsunoda},
  journal={Cloning and stem cells},
  year={2003},
  volume={5 4},
  pages={
          379-87
        }
}
Brief treatment of metaphase II (MII) stage porcine oocytes with 0.4 microg/mL demecolcine in the presence of 0.05 M sucrose produces a membrane protrusion that contains a condensed chromosome mass. The present study examined the optimal conditions for demecolcine and nocodazole treatment in chemically assisted removal of chromosomes. When matured oocytes were treated with 0.1-0.4 microg/mL demecolcine for 60 min or with 0.4 microg/mL demecolcine for 30 min or 3 microg/mL nocodazole for 30 or… CONTINUE READING
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