Effect of cytokinins and lines on plant regeneration from long-term callus and suspension cultures of Allium cepa L.

@article{Zheng2004EffectOC,
  title={Effect of cytokinins and lines on plant regeneration from long-term callus and suspension cultures of Allium cepa L.},
  author={Sijun Zheng and Betty Henken and Eri Sofiari and Paul L. C. Keizer and Evert Jacobsen and Chris Kik and Frans A. Krens},
  journal={Euphytica},
  year={2004},
  volume={108},
  pages={83-90}
}
Suspension cultures were initiated from callus cultures of Allium cepa, which had been precultured on a solidified medium for 7 months. For another 3 months the 83 callus lines were kept in suspension culture. Each line is derived from a single zygotic embryo, 24 lines from onion and 59 lines from shallot. Of these, 20 suspension lines showed adequate growth and were used to test the effect of cytokinins on plant regeneration. On average, 1.25% of the calli produced shoots on a growth regulator… CONTINUE READING
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