Effect of cysteine desulfhydrase gene disruption on l-cysteine overproduction in Escherichia coli

@article{Awano2003EffectOC,
  title={Effect of cysteine desulfhydrase gene disruption on l-cysteine overproduction in Escherichia coli},
  author={Naoki Awano and Masaru Wada and A. Kohdoh and Tadao Oikawa and Hiroshi Takagi and Shigeru Nakamori},
  journal={Applied Microbiology and Biotechnology},
  year={2003},
  volume={62},
  pages={239-243}
}
In Escherichia coli, the enzyme called cysteine desulfhydrase (CD), which is responsible for l-cysteine degradation, was investigated by native-PAGE and CD activity staining of crude cell extracts. Analyses with gene-disrupted mutants showed that CD activity resulted from two enzymes: tryptophanase (TNase) encoded by tnaA and cystathionine β-lyase (CBL) encoded by metC. It was also found that TNase synthesis was induced by the presence of l-cysteine. The tnaA and metC mutants transformed with… CONTINUE READING

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