Effect of cycloheximide on the synthesis and processing of 5S RNA in HeLa cells.

The synthesis and incorporation into ribosomes of 5S RNA were investigated under conditions of protein synthesis inhibition by cycloheximide. Within 1 h after addition of cycloheximide to the culture medium, the synthesis of 5S RNA in HeLa cells, as measured by the pulse labeling of total 5S RNA, was inhibited by 25-30 per cent, but the degree of inhibition… (More)