Effect of cryoprotectants on the survival of follicles in frozen mouse ovaries.

@article{Candy1997EffectOC,
  title={Effect of cryoprotectants on the survival of follicles in frozen mouse ovaries.},
  author={C J Candy and Malissa J Wood and David G Whittingham},
  journal={Journal of reproduction and fertility},
  year={1997},
  volume={110 1},
  pages={11-9}
}
Ovaries from 10-day-old mice were exposed to 1.5 mol l-1 dimethylsulfoxide, 1,2-propanediol, ethanediol or glycerol for 5-60 min at room temperature before freezing. Follicles in fresh and frozen ovaries were counted and scored as normal or damaged in stained serial sections. More primordial follicles survived in ovaries frozen in dimethylsulfoxide, 1,2-propanediol and ethanediol (81-94%) than in those frozen in glycerol (4-28%). Prolonged exposure to ethanediol (60 min) before cooling… CONTINUE READING

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