Effect of cryoprotectants on release of various enzymes from buck spermatozoa during freezing.

@article{Sirat1996EffectOC,
  title={Effect of cryoprotectants on release of various enzymes from buck spermatozoa during freezing.},
  author={M P Sirat and Arabinda K. Sinha and Birendra Kumar Singh and R. L. N. Sai Prasad},
  journal={Theriogenology},
  year={1996},
  volume={45 2},
  pages={405-16}
}
Semen samples from 12 bucks Were extended with 10 different extenders containing glycerol, DMSO, glycerol + DMSO, and glycerol + lactose in varying concentrations as cryoprotective agents. The activities of acrosin, hyaluronidase, alkaline phosphatase (AKP), aspartate aminotransferase (AST), alanine amino transferase (ALT) and lactic dehydrogenase (LDH) were assayed in equilibrated (Prefreeze) and frozen thawed (Postfreeze) semen samples. Significantly (P < 0.01) higher intracellular activity… CONTINUE READING

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