Effect of cryopreservation on apoptotic-like events and its relationship with cryocapacitation of buffalo (Bubalus bubalis) sperm.

@article{Kadirvel2012EffectOC,
  title={Effect of cryopreservation on apoptotic-like events and its relationship with cryocapacitation of buffalo (Bubalus bubalis) sperm.},
  author={Govindasamy Kadirvel and Srinivasan Periasamy and Satish Kumar},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2012},
  volume={47 1},
  pages={143-50}
}
This study investigated the apoptosis-like events associated with cryopreservation process and their relationship with cryocapacitation in buffalo (Bubalus bubalis) sperm. A total of 49 semen ejaculates from seven bulls were studied for structural changes in sperm following cryopreservation. Apoptotic changes were detected by assays specific for translocation of phosphatidylserine (PS) to the cell surface, alterations in membrane permeability and mitochondrial membrane potential (MMP), and DNA… CONTINUE READING

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Apoptotic changes were detected by assays specific for translocation of phosphatidylserine ( PS ) to the cell surface , alterations in membrane permeability and mitochondrial membrane potential ( MMP ) , and DNA integrity .
Apoptotic changes were detected by assays specific for translocation of phosphatidylserine ( PS ) to the cell surface , alterations in membrane permeability and mitochondrial membrane potential ( MMP ) , and DNA integrity .
A significant ( p < 0.01 ) percentage of cryopreserved sperm showed externalization of PS and early apoptotic changes and lowered MMP when compared with the fresh sperm .
A significant ( p < 0.01 ) percentage of cryopreserved sperm showed externalization of PS and early apoptotic changes and lowered MMP when compared with the fresh sperm .
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