[Effect of chronic exposure to nitrogen dioxide on biogenic amine levels in the brain of guinea pigs].

Abstract

The experiment was carried out on male guinea pigs exposed to NO2 at a constant concentration of 0.1 ppm 24 h daily for 1, 2.5 and 6 months, or NO2 concentration was increased to 0.5 ppm twice daily, between 7:00 and 8:00 AM and 4:00 and 5:00 PM. After the exposure to NO2 was over the animals were killed by decapitation. The cranium was opened and the brain removed, and afterwards at 0 degrees C the left hemisphere was dissected into discrete areas which were then frozen on dry ice and weighed. The tissues were kept for 2-3 days at -20 degrees C, and afterwards the content of noradrenaline, 5-hydroxytryptamine and 5-hydroxyindole-acetic acid was estimated spectrofluorometrically in examined parts of the brain (thalamus--hypothalamus pons + medulla oblongata hippocampus, cortex frontalis). It was shown that the applied NO2 concentrations brought about slight and nonspecific alterations in the content of the investigated amines in guinea pigs' brain.

Cite this paper

@article{Feliska1985EffectOC, title={[Effect of chronic exposure to nitrogen dioxide on biogenic amine levels in the brain of guinea pigs].}, author={W Felińska and Ryszard Szkilnik and B Stanosek and Mary Fran Kaminski and K Ludyga and Ryszard Brus}, journal={Medycyna pracy}, year={1985}, volume={36 4}, pages={236-43} }