Effect of chromosomal position on amplification of transfected genes in animal cells

@article{Wahl1984EffectOC,
  title={Effect of chromosomal position on amplification of transfected genes in animal cells},
  author={G. Wahl and B. Vincent and M. DeRose},
  journal={Nature},
  year={1984},
  volume={307},
  pages={516-520}
}
  • G. Wahl, B. Vincent, M. DeRose
  • Published 1984
  • Biology, Medicine
  • Nature
    • A single protein (CAD) contains the first three enzymatic activities of de novo undine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one transformant is 100-fold that of the others; in another, amplification of donated genes inserted near a centromere results in chromosome instability and rearrangements. 
    View on Springer
    ncbi.nlm.nih.gov
    98 Citations
    Background Citations
    13

    Topics from this paper.

    Explore Further: Topics Discussed in This Paper

      98 Citations
      Citation Type

      Citation Type

      The role of acentric chromosome fragments in gene amplification
      • 23
      Effect of genomic position on amplification of the DFR1 gene in Saccharomyces cerevisiae.
      • 5
      Plasmid amplification-promoting sequences from the origin region of Chinese hamster dihydrofolate reductase gene do not promote position-independent chromosomal gene amplification
      • 5
      Site-specific integration of H-ras in transformed rat embryo cells.
      • 28
      Localization of a cis-acting element responsible for the developmentally regulated amplification of drosophila chorion genes
      • 82
      Association of high rate of recombination with amplification of dominant selectable gene in human cells
      • 14
      Influence of cellular sequences on instability of plasmid integration sites in human cells
      • J. Murnane
      • Biology, Medicine
      • Somatic cell and molecular genetics
      • 1990
      • 7
      Amplification and Molecular Cloning of Transfected Genes
      • 1
      Genetic alterations of integrated avian sarcoma virus DNA sequences in transformed rat cells.
      • 2
      High-level expression of foreign genes in mammalian cells.
      • S. Kane
      • Biology, Medicine
      • Genetic engineering
      • 1991
      • 10

      References

      SHOWING 1-10 OF 41 REFERENCES
      The role of gene dosage and genetic transpositions in carcinogenesis
      • 594
      The cloning and reintroduction into animal cells of a functional CAD gene, a dominant amplifiable genetic marker
      • 68
      Evolution of chromosomal regions containing transfected and amplified dihydrofolate reductase sequences.
      • 83
      • PDF
      Transforming DNA integrates into the host chromosome
      • 211
      Single-copy and amplified CAD genes in Syrian hamster chromosomes localized by a highly sensitive method for in situ hybridization.
      • 60
      • PDF
      DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
      • 847
      • PDF
      Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells.
      • 292
      Structure of the gene for CAD, the multifunctional protein that initiates UMP synthesis in Syrian hamster cells.
      • 47
      • PDF
      Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.
      • 252
      • PDF
      Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae
      • 340