Effect of chromosomal position on amplification of transfected genes in animal cells
@article{Wahl1984EffectOC, title={Effect of chromosomal position on amplification of transfected genes in animal cells}, author={G. Wahl and B. Vincent and M. DeRose}, journal={Nature}, year={1984}, volume={307}, pages={516-520} }
A single protein (CAD) contains the first three enzymatic activities of de novo undine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one transformant is 100-fold that of the others; in another, amplification of donated genes inserted near a centromere results in chromosome instability and rearrangements.
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References
SHOWING 1-10 OF 41 REFERENCES
The cloning and reintroduction into animal cells of a functional CAD gene, a dominant amplifiable genetic marker
- Biology, Medicine
- Cell
- 1981
- 69
Evolution of chromosomal regions containing transfected and amplified dihydrofolate reductase sequences.
- Biology, Medicine
- Molecular and cellular biology
- 1983
- 83
- PDF
Single-copy and amplified CAD genes in Syrian hamster chromosomes localized by a highly sensitive method for in situ hybridization.
- Biology, Medicine
- Molecular and cellular biology
- 1982
- 60
- PDF
DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
- Biology, Medicine
- Proceedings of the National Academy of Sciences of the United States of America
- 1979
- 858
- PDF
Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells.
- Biology, Medicine
- The Journal of biological chemistry
- 1979
- 292
Structure of the gene for CAD, the multifunctional protein that initiates UMP synthesis in Syrian hamster cells.
- Biology, Medicine
- Molecular and cellular biology
- 1982
- 48
- PDF
Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.
- Biology, Medicine
- Proceedings of the National Academy of Sciences of the United States of America
- 1978
- 254
- PDF
Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae
- Biology, Medicine
- Nature
- 1980
- 344