Effect of cholesterol on the motility and plasma membrane integrity of frozen equine spermatozoa after thawing.

@article{Combes2000EffectOC,
  title={Effect of cholesterol on the motility and plasma membrane integrity of frozen equine spermatozoa after thawing.},
  author={G{\'e}raldine Combes and Dickson D Varner and Friedhelm Schroeder and Robert C. Burghardt and Terry L. Blanchard},
  journal={Journal of reproduction and fertility. Supplement},
  year={2000},
  volume={56},
  pages={127-32}
}
The aim of the present study was to investigate the cryoprotectant properties of cholesterol after incorporation into the plasma membranes of equine spermatozoa. A cholesterol-methyl-beta-cyclodextrin complex was used to alter sperm plasma membrane cholesterol content. Ejaculates from six stallions were centrifuged in a non-fat skimmed milk glucose-sucrose extender (MK) or a modified Tyrode's medium (TALP). The sperm pellets were resuspended in the appropriate extender with or without added… CONTINUE READING