Effect of chitosan malate on viability and cytoskeletal structures morphology of Caco-2 cells.

  title={Effect of chitosan malate on viability and cytoskeletal structures morphology of Caco-2 cells.},
  author={Claudia Juliano and Grazia Galleri and Therese Klemetsrud and Jan Karlsen and Paolo Giunchedi},
  journal={International journal of pharmaceutics},
  volume={420 2},
The aim of this work was to evaluate the effects of the treatment with chitosan malate (CM) on viability of Caco-2 cells and on the morphology and the integrity of their cytoskeletal structures (microtubules, microfilaments). Cytotoxicity of CM, both as a solution and as microparticles obtained by spray drying, was evaluated by using the reduction of MTT reagent; microtubule and microfilaments organization of Caco-2 cells treated with CM solution was examined with immunofluorescence techniques… 
Polymer coated liposomes for use in the oral cavity – a study of the in vitro toxicity, effect on cell permeability and interaction with mucin
Although the chitosan-coated liposomes affected the cell viability, this formulation also influenced the cell permeability, which makes it an interesting candidate for systemic drug delivery from the oral cavity.
Dual effect of F-actin targeted carrier combined with antimitotic drug on aggressive colorectal cancer cytoskeleton: Allying dissimilar cell cytoskeleton disrupting mechanisms.
This study suggests that employing such a suitable pair of drug-carriers with dissimilar sites of action, thus allying the different cell cytoskeleton disrupting mechanisms, may provide a more efficient cancer therapy approach.
Intracellular ion and protein nanoparticle-induced osmotic pressure modify astrocyte swelling and brain edema in response to glutamate stimuli
It is suggested that intracellular osmotic pressure (especially PN-OP) has a pivotal role in glutamate-induced astrocyte swelling and brain edema and recovery of cytoplasmic potential is a promising target to develop new drugs and curebrain edema.
Buccal Delivery of Nanoparticles
The buccal route offers an alternative for drug administration due to its advantages, including the avoidance of the gastrointestinal tract, the hepatic first-pass, enzymatic degradation and chemical


Effect of chitosan on epithelial permeability and structure.
The data suggest that chitosan increases cell permeability by affecting paracellular and intracellular pathways of epithelial cells, in a reversible manner.
Hydrogen peroxide-induced cytotoxicity in cultured epithelial cells (WISH): A functional and morphological study.
Investigation of the effect of hydrogen peroxide on cultured epithelial cells (WISH) revealed changes in microtubules and microfilaments, which are two of the main cytoskeletal components.
Chitosan derivatives killed bacteria by disrupting the outer and inner membrane.
Results revealed that the antimicrobial action of water-soluble chitosans was dependent on the degree of deacetylation and the substituted group, and these results revealed that dimethylaminoethyl-chitosan has the highest potential to suppress the growth of bacteria.
Effect of Chitosan on Epithelial Cell Tight Junctions
Chitosan-mediated tight junction disruption is caused by a translocation of tight junction proteins from the membrane to the cytoskeleton, causing a reduction in transepithelial electrical resistance of Caco-2 monolayers.
The effect of chitosan and other polycations on tight junction permeability in the human intestinal Caco-2 cell line(1).
The results indicate that oral ingestion of chitosan may have more widespread health effects by altering intestinal barrier function, thus allowing the entrance into the circulation of potentially toxic and/or allergenic substances.
The effect of topology of chitosan biomaterials on the differentiation and proliferation of neural stem cells.
Observations indicate that chitosan topology can play an important role in regulating differentiation and proliferation of NSCs and raise the possibility of the utilization of chitOSan in various structural biomaterials in neural tissue engineering.
Evaluation of the biological properties of soluble chitosan and chitosan microspheres
Chitosan is the N-deacetylated product of chitin, a naturally occurring polymer which has been used extensively to prepare microspheres for oral and intra-nasal delivery. The chitosan polymer has
Topical formulations and wound healing applications of chitosan.
Chitosan enhances the functions of inflammatory cells such as polymorphonuclear leukocytes (PMN), phagocytosis, production of osteopontin and leukotriene B4, macrophages, and fibroblasts, which promotes granulation and organization, therefore chitosans is beneficial for the large open wounds of animals.
Chitosan microparticles containing plasmid DNA as potential oral gene delivery system.
  • U. Guliyeva, F. Oner, S. Ozsoy, R. Haziroğlu
  • Biology, Medicine
    European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
  • 2006
The potential of chitosan as a polycationic gene carrier for oral administration has been explored since 1990s. Chitosan has been shown to effectively bind DNA in saline or acetic acid solution and
Chitosan—A versatile semi-synthetic polymer in biomedical applications
This review outlines the new developments on chitosan-based bioapplications. Over the last decade, functional biomaterials research has developed new drug delivery systems and improved scaffolds for