Effect of cellular aging on collagen biosynthesis: II. Collagen synthesis and deposition by a human skin fibroblast strain over 25 passages.


Collagen synthesis and accumulation were studied by serial cultures of human dermal fibroblasts. A freshly seeded strain of cells was compared to cryopreserved cells from the same donor. Up to 25 passages there was no clear sign of reaching phase III, the decline of the culture. This was ascertained by the count of the dead cells floating on top of the… (More)