Effect of cations and temperature on kinetics of desmin assembly.

  title={Effect of cations and temperature on kinetics of desmin assembly.},
  author={Marvin H. Stromer and Mary A. Ritter and Y Y Pang and Richard M. Robson},
  journal={The Biochemical journal},
  volume={246 1},
Smooth-muscle desmin, which was isolated from avian gizzard, was purified and used to form reconstituted intermediate filaments. Filament assembly was done in the presence of physiological cations, Ca2+, Mg2+, Na+, and Na+ plus Mg2+, and with non-physiological cations Cu2+ and Ni2+. Assembly was done at 2 degrees, 22 degrees and 37 degrees C, and was monitored by absorbance and by electron microscopy. Absorbance increased most rapidly during the first 2-5 min and then increased at a slower rate… CONTINUE READING

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