Effect of carrageenans alone and in combination with casein or lipopolysaccharide on human epithelial intestinal HT-29 cells.
@article{Sokolova2017EffectOC, title={Effect of carrageenans alone and in combination with casein or lipopolysaccharide on human epithelial intestinal HT-29 cells.}, author={Ekaterina V Sokolova and A. S. Kuz′mich and Anna O. Byankina and Irina M. Yermak}, journal={Journal of biomedical materials research. Part A}, year={2017}, volume={105 10}, pages={ 2843-2850 } }
The research described here was focused on the effect on human intestinal epithelial cell monolayers of sulfated red algal polysaccharides (κ-, λ-, and κ/β-carrageenans) alone and in combination with casein or lipopolysaccharide (LPS). HT-29 cells were investigated under normal and stress conditions; stress was induced by exposure to ethanol. Cell viability was monitored with a real-time system. The change in binding properties of negatively sulfated red algal polysaccharides assessed by the…
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References
SHOWING 1-10 OF 41 REFERENCES
Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines.
- Biology, MedicineFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- 2016
Effects of structural peculiarities of carrageenans on their immunomodulatory and anticoagulant activities.
- BiologyCarbohydrate polymers
- 2012
Casein phosphopeptides modulate proliferation and apoptosis in HT-29 cell line through their interaction with voltage-operated L-type calcium channels.
- BiologyThe Journal of nutritional biochemistry
- 2012
Probiotics inhibit enteropathogenic E. coliadherence in vitro by inducing intestinal mucin gene expression.
- Biology, MedicineAmerican journal of physiology. Gastrointestinal and liver physiology
- 1999
The hypothesis is that the ability of probiotic agents to inhibit adherence of attaching and effacing organisms to intestinal epithelial cells is mediated through their ability to increase expression of MUC2 and MUC3 intestinal mucins.
Food additive carrageenan: Part II: A critical review of carrageenan in vivo safety studies
- BiologyCritical reviews in toxicology
- 2014
Based on the many animal subchronic and chronic toxicity studies, CGN has not been found to affect the immune system, as judged by lack of effects on organ histopathology, clinical chemistry, hematology, normal health, and the lack of target organ toxicities.
The common food additive carrageenan is not a ligand for Toll-Like- Receptor 4 (TLR4) in an HEK293-TLR4 reporter cell-line model.
- BiologyFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- 2015
Mechanisms of cross hyporesponsiveness to Toll-like receptor bacterial ligands in intestinal epithelial cells.
- Biology, MedicineGastroenterology
- 2004
Continuous presence of specific bacterial components results in a status of hyporesponsiveness in otherwise reactive IEC, and down-regulation of TLR surface expression and up- regulation of inhibitory Tollip with decreased phosphorylation of IRAK might all contribute to this hyporeSponsiveness.
Conformational changes in iota- and kappa-carrageenans induced by complex formation with bovine beta-casein.
- ChemistryBiomacromolecules
- 2007
The interaction of beta-casein with iota- and kappa-carrageenans was approximated in terms of the model of binding of large ligands to macromolecules, that provides the binding constants for these biopolymers.
Casein phosphopeptide promotion of calcium uptake in HT‐29 cells − relationship between biological activity and supramolecular structure
- Chemistry, BiologyThe FEBS journal
- 2007
Casein phosphopeptides (CPPs) form aggregated complexes with calcium phosphate and induce Ca2+ influx into HT‐29 cells that have been shown to be differentiated in culture. The relationship between…
Decreased Expression of Toll-Like Receptor-4 and MD-2 Correlates with Intestinal Epithelial Cell Protection Against Dysregulated Proinflammatory Gene Expression in Response to Bacterial Lipopolysaccharide1
- BiologyThe Journal of Immunology
- 2001
It is concluded that IEC limit dysregulated LPS signaling by down-regulating expression of MD-2 and TLR4 and the remainder of the intracellular L PS signaling pathway is functionally intact.