Effect of carrageenans alone and in combination with casein or lipopolysaccharide on human epithelial intestinal HT-29 cells.

  title={Effect of carrageenans alone and in combination with casein or lipopolysaccharide on human epithelial intestinal HT-29 cells.},
  author={Ekaterina V Sokolova and A. S. Kuz′mich and Anna O. Byankina and Irina M. Yermak},
  journal={Journal of biomedical materials research. Part A},
  volume={105 10},
The research described here was focused on the effect on human intestinal epithelial cell monolayers of sulfated red algal polysaccharides (κ-, λ-, and κ/β-carrageenans) alone and in combination with casein or lipopolysaccharide (LPS). HT-29 cells were investigated under normal and stress conditions; stress was induced by exposure to ethanol. Cell viability was monitored with a real-time system. The change in binding properties of negatively sulfated red algal polysaccharides assessed by the… 

Effects of Carrageenans on Biological Properties of Echinochrome

It was showed that CRGs significantly decreased the spermotoxicity of Ech, against the sea urchin S. intermedius sperm, and the Ech’s protective action towards human epithelial HT-29 cells remains to be unaltered in the complex, with κ/β-CRG, under stress conditions.

Determination of carrageenan in jellies with new methylene blue dye using spectrophotometry, smartphone-based colorimetry and spectrophotometric titration

Carrageenan is a seaweed-derived sulfated polysaccharide known as food additive E407. It is used as gelling and thickening agent in cheeses, puddings, desserts and cured meats (Bednářová et al.,



Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines.

Probiotics inhibit enteropathogenic E. coliadherence in vitro by inducing intestinal mucin gene expression.

The hypothesis is that the ability of probiotic agents to inhibit adherence of attaching and effacing organisms to intestinal epithelial cells is mediated through their ability to increase expression of MUC2 and MUC3 intestinal mucins.

Food additive carrageenan: Part II: A critical review of carrageenan in vivo safety studies

  • M. Weiner
  • Biology
    Critical reviews in toxicology
  • 2014
Based on the many animal subchronic and chronic toxicity studies, CGN has not been found to affect the immune system, as judged by lack of effects on organ histopathology, clinical chemistry, hematology, normal health, and the lack of target organ toxicities.

The common food additive carrageenan is not a ligand for Toll-Like- Receptor 4 (TLR4) in an HEK293-TLR4 reporter cell-line model.

Mechanisms of cross hyporesponsiveness to Toll-like receptor bacterial ligands in intestinal epithelial cells.

Continuous presence of specific bacterial components results in a status of hyporesponsiveness in otherwise reactive IEC, and down-regulation of TLR surface expression and up- regulation of inhibitory Tollip with decreased phosphorylation of IRAK might all contribute to this hyporeSponsiveness.

Conformational changes in iota- and kappa-carrageenans induced by complex formation with bovine beta-casein.

The interaction of beta-casein with iota- and kappa-carrageenans was approximated in terms of the model of binding of large ligands to macromolecules, that provides the binding constants for these biopolymers.

Casein phosphopeptide promotion of calcium uptake in HT‐29 cells − relationship between biological activity and supramolecular structure

Casein phosphopeptides (CPPs) form aggregated complexes with calcium phosphate and induce Ca2+ influx into HT‐29 cells that have been shown to be differentiated in culture. The relationship between

Decreased Expression of Toll-Like Receptor-4 and MD-2 Correlates with Intestinal Epithelial Cell Protection Against Dysregulated Proinflammatory Gene Expression in Response to Bacterial Lipopolysaccharide1

It is concluded that IEC limit dysregulated LPS signaling by down-regulating expression of MD-2 and TLR4 and the remainder of the intracellular L PS signaling pathway is functionally intact.