Effect of buffer components and carrier solvents on in vitro activity of recombinant human carboxylesterases.

@article{Williams2008EffectOB,
  title={Effect of buffer components and carrier solvents on in vitro activity of recombinant human carboxylesterases.},
  author={Eric T Williams and Mariam E. Ehsani and Xi-liang Wang and He Wang and Yue-wei Qian and Steven A. Wrighton and Everett J Perkins},
  journal={Journal of pharmacological and toxicological methods},
  year={2008},
  volume={57 2},
  pages={138-44}
}
INTRODUCTION The effects of buffer and substrate solvent conditions on in vitro activity of carboxylesterases (CE) have not been previously described. Therefore, it is unknown if the many different assay conditions used by various laboratories have a substantial impact on the activity of CE enzymes. METHODS Three human CEs were expressed and purified, and the hydrolysis of 4-nitrophenyl butyrate was measured to assess enzyme activity. Four buffers (HEPES, potassium phosphate, sodium phosphate… CONTINUE READING