Corpus ID: 6340572

Effect of brimonidine on rabbit trabecular meshwork hyaluronidase activity.

  title={Effect of brimonidine on rabbit trabecular meshwork hyaluronidase activity.},
  author={Jorge Benozzi and Carolina O. Jaliffa and F Firpo Lacoste and D W Llomovatte and Mar{\'i}a In{\'e}s Keller Sarmiento and Ruth E. Rosenstein},
  journal={Investigative ophthalmology \& visual science},
  volume={41 8},
PURPOSE To study the presence of hyaluronidase activity in the rabbit trabecular meshwork and its regulation by brimonidine. METHODS A spectrophotometric assay that consists of the assessment of N-acetylhexosamine groups released from hyaluronic acid was used to examine hyaluronidase activity. Cyclic adenosine monophosphate (cAMP) levels were assessed by radioimmunoassay. RESULTS Hyaluronidase activity was detected in the rabbit trabecular meshwork. Its optimal activity was in the acid… Expand
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Hyaluronidase in Ocular Tissues *
The spreading reaction is a biological method, measuring the decomposing effect of hyaluronidase on the mucoids of the cement substance of rabbit's skin, and the chemical method consists in measuring the increase in reducing sugar, or in liberated acetyl-glucosamine after depolymerized hyaluronic acid. Expand
Hyaluronan in the human trabecular meshwork.
Pronounced HA staining observed in the various layers of the trabecular meshwork suggests that substantial amount of HA is present in the nonglaucomatous outflow pathway and supports potential roles for this glycosaminoglycan in the regulation of the physiological aqueous out flow resistance or in the maintenance of the outflow channels or both. Expand
Human vitreous hyaluronidase: isolation and characterization.
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Cultured human trabecular meshwork cells express functional alpha 2A adrenergic receptors.
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Exogenous hyaluronidases and degradation of hyaluronic acid in the rabbit eye.
The results of these studies indicate that SH is more effective than TH in decreasing aqueous outflow resistance and that hyaluronic acid is an important GAG contributor to aqueously out flow resistance in the normal rabbit eye. Expand
Corneal Endothelial Hyaluronidase: A Role in Anterior Chamber Hyaluronic Acid Catabolism
Human corneal endothelial hyaluronidase may play a role in normal anterior segment HA metabolism and in the degradation of highly concentrated HA used as a viscoelastic. Expand
Production of glycosaminoglycans by cell cultures of the trabecular meshwork of the primate eye.
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Trabecular meshwork cell culture in glaucoma research: evaluation of biological activity and structural properties of human trabecular cells in vitro.
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Turnover of hyaluronan in the tissues
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Trabecular meshwork glycosaminoglycans in human and cynomolgus monkey eye.
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