Corpus ID: 24661427

Effect of auto-oxidation products from cholesterol on aortic smooth muscle cells: an in vitro study.

  title={Effect of auto-oxidation products from cholesterol on aortic smooth muscle cells: an in vitro study.},
  author={S. Peng and C. B. Taylor and P. Thắm and N. Werthessen and B. Mikkelson},
  journal={Archives of pathology \& laboratory medicine},
  volume={102 2},
Purified cholesterol is quite unstable when stored in air at room temperature. Products of cholesterol auto-oxidation were concentrated from several lots of USP-grade cholesterol by recrystallizing cholesterol from the methanol extract, retaining the mother liquor, and evaporating the residuum to dryness under vacuum. By application of thin-layer chromatography and gas-liquid chromatography, major, individual, oxidation compounds were identified and quantitated. Biological activities of these… Expand
Influence of Cholesterol Oxidation Derivatives on Membrane Bound Enzymes in Cultured Aortic Smooth Muscle Cells 1
  • S. Peng, J. Hill, R. Morin, C. B. Taylor
  • Chemistry, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1985
It was demonstrated that 5′-nucleotidase activity was also markedly decreased in plasma membrane-enriched fractions when cells were incubated with cholestane-3β,5α,6β-triol, and Na+, K+-ATPase activity in crude membranes showed a significant decrease. Expand
Analysis for and Generation of Cholesterol Oxidation Products in Egg Yolk by Heat Treatment
Abstract Certain oxidation products of cholesterol exhibit a much greater biopotency for inducing atherogenesis, cell cytotoxicity, and inhibiting cholesterol biosynthesis than cholesterol. EarlyExpand
Transport of cholesterol autoxidation products in rabbit lipoproteins.
Radioactivities of each fraction in lipoproteins separated by thin layer chromatography showed that fractions containing cholestane-3 beta,5 alpha,6 beta-triol, 7 alpha- and 7 beta-hydroxycholesterol and 7-ketocholesterol were more selectively transported in VLDL, whereas most of the 25-hydxycholesterol was present in LDL, while HDL contained only minute amounts of cholesterol oxidation products. Expand
Effects of cholesterol autoxidation derivatives on hexose transport in cultured aortic smooth muscle cells.
25-Hydroxycholesterol, a potent inhibitor of sterol biosynthesis in cultured arterial smooth muscle cells, did not affect hexose transport up to 8 hr of incubation, and the cytotoxic effect of 25-hydroxych cholesterol, therefore, may be a consequence of the reduced sterolsynthesis caused by this sterol. Expand
Reducing the degree of lipoid infiltration of rabbit tissues by feeding cholesterol freed from its oxidation products
It was interesting to study lipid deposition in the tissues of animals during chronic peroral administration of the ChS preparations having undergone different degrees of oxidation. Expand
Connective tissue responses to oxysterols.
The triol was most active in its cytopathic effect on cells in culture, in size of granuloma formation and in producing necrosis, and the epoxide, 25-hydroxycholesterol and air-oxidized cholesterol produced rather less effect on tissues and cultures than the triol, but more than purified cholesterol and the control. Expand
Cytotoxic Mechansims for Cholesterol Oxidation Products in Fibroblasts and Endothelial Cells
Several cholesterol oxides form during free radical oxidation of membrane lipids. These cholesterol oxides have been recovered from tissues and at least eight of these are products consistentlyExpand
Distribution of 25-hydroxycholesterol in plasma lipoproteins and its role in atherogenesis. A study in squirrel monkeys.
Oxidation products of cholesterol have been shown to be potent inhibitors of cholesterol biosynthesis and also highly toxic to cultured aortic smooth muscle cells and the possibility that plasma lipoproteins containing beta-apoprotein (i.e. LDL and VLDL) may carry more oxidation products than HDL which is not atherogenic. Expand
[Quantitative analysis of auto-oxidation products of cholesterol in food of animal origin].
After extraction with methylene chloride, isolation of the unsaponifiable lipid fraction and enrichment by two-step column chromatography, the oxycholesterols were gas chromatographically separatedExpand
Analysis and physiologic significance of cholesterol epoxide in animal tissues
Even though the case for direct involvement of cholesterol epoxide in the etiology of carcinogenesis or other diseases is equivocal, the epoxide or its metabolites may yet prove valuable as a diagnostic aid in revealing abnormal function associated with certain diseases. Expand