• Corpus ID: 13849149

Effect of apoptosis inducing factors on artesunate-induced astrocytic glioma apoptosis

@inproceedings{Liu2017EffectOA,
  title={Effect of apoptosis inducing factors on artesunate-induced astrocytic glioma apoptosis},
  author={Bin Liu and Junhua Yu},
  year={2017}
}

• B. Liu, Junhua Yu
• Published 2017
• Biology

Objective: To explore the effect of apoptosis inducing factors (AIFs) on artesunate (ART)-induced apoptosis in astrocytic glioma. Methods: CCK-8 Kit was used to detect the viability of U-87 MG cells treated with ART at different concentrations and different processing time. ART-induced cell death was detected by nuclear staining followed by flow cytometry. The effect of ART on mitochondrial membrane potential was determined by Rhodamine123 staining. AIF was then silenced by RNA interference, the effect of ART on cell viability was detected by CCK-8 kit and then its effect on the expression of AIF was measured by Western blot. An ectopic glioma model was established in nude mice and the in vivo anti-tumor effect of ART was investigated. Results: ART decreased the viability of cells to various degrees, which was concentrationand time-dependent. ART induced apoptosis and led to the decline of mitochondrial membrane potential. A dose of 40 ug/ml ART induced 58.5 ± 2.9% of apoptosis rate and 61.7% decline of mitochondrial membrane potential. After AIF was silenced, ART-induced cell viability was increased and ART-induced expression of AIF was decreased. In vivo anti-tumor experiment indicated that ART could inhibit the growth of glioma effectively. Conclusion: ART can induce mitochondria-mediated apoptosis in U87 cells and AIFs are involved in ART-induced apoptosis.