Effect of anti-freeze protein (AFP) on the cooling and freezing of equine embryos as measured by DAPI-staining.

@article{Lagneaux1997EffectOA,
  title={Effect of anti-freeze protein (AFP) on the cooling and freezing of equine embryos as measured by DAPI-staining.},
  author={Delphine Lagneaux and Mika Huhtinen and Eric Koskinen and Elisabeth Palmer},
  journal={Equine veterinary journal. Supplement},
  year={1997},
  volume={25},
  pages={85-7}
}
Equine embryos recovered on Day 6 after ovulation were cooled to +4 degrees C, or frozen with AFP alone or together with glycerol. Twenty embryos (140-200 microm in diameter) were randomly assigned to 6 treatment groups. In the first 3 groups, the embryos were cooled from room temperature to +4 degrees C at a rate of 3 degrees C/min and warmed again at a rate of 32 degrees C/min in a programmable freezer. In the second 3 groups, the embryos were frozen using a standard protocol, stored in… CONTINUE READING