Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts.

@article{Uemura1990EffectOA,
  title={Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts.},
  author={Kazumasa Uemura and Eiko Sugiyama and Chie Tamai and Atsushi Hara and Tamotsu Taketomi and N. S. Radin},
  journal={Journal of biochemistry},
  year={1990},
  volume={108 4},
  pages={525-30}
}
1-Phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), an effective inhibitor of UDP-glucose:ceramide glucosyltransferase, caused growth inhibition of cultured rabbit skin fibroblasts in a dose-dependent manner. At 50 microM both threo and erythro isomers of PDMP completely suppressed the cell growth. Major gangliosides of the fibroblasts, GM3 and GD3, were greatly reduced in amounts in the presence of threo-PDMP and accumulation of ceramides was observed. Surface labeling with galactose… CONTINUE READING