Effect of adrenaline and glucocorticoids on monocyte cAMP-specific phosphodiesterase (PDE4) in a monocytic cell line

  title={Effect of adrenaline and glucocorticoids on monocyte cAMP-specific phosphodiesterase (PDE4) in a monocytic cell line},
  author={Mercedes Delgado and Soledad M. Fern{\'a}ndez-Alfonso and Jos{\'e} Angel Fuentes},
  journal={Archives of Dermatological Research},
An increase in phosphodiesterase 4 (PDE4) in blood mononuclear white cells of patients with atopic dermatitis (AD) has been described. This and other skin disorders worsen during stress, during which there are increased circulating levels of adrenaline and glucocorticoids. The aim of this study was to analyse the effect of both these hormones on PDE4 in inflammatory cells. The human monocyte cell line U-937 was used as a model of blood mononuclear leucocytes. For this purpose, (1) the effect of… Expand
Effect of β-agonist on the dexamethasone-induced expression of aromatase by the human monocyte cells
Results may suggest that attenuation of estrogen secretion from peripheral monocyte-derived cell line THP-1 cells could be a part of the pathology of stress-caused deterioration of atopic dermatitis. Expand
Adrenaline induced disruption of endogenous melatoninergic system, antioxidant and inflammatory responses in the gastrointestinal tissues of male Wistar rat: an in vitro study
      The current study aimed to demonstrate the potentially adverse effects of adrenaline, an endogenous stressor, on the melatonergic system, oxidative status, antioxidative responses andExpand
Resolution-phase macrophages possess a unique inflammatory phenotype that is controlled by cAMP
It transpires that although rMs are dispensable for clearing PMNs during self-limiting inflammation, they are essential for signaling postresolution lymphocyte repopulation via COX 2 lipids, with a role in mediating postresolution innate-lymphocyteRepopulation and restoring tissue homeostasis. Expand
Curcuma longa extract protects against gastric ulcers by blocking H2 histamine receptors.
Findings suggest that the extract from C. longa specifically inhibits gastric acid secretion by blocking H(2) histamine receptors in a competitive manner. Expand
Stress and Immunity
There is definitely a brain–skin connection that can translate emotional stressors into biochemical mediators that can result in adverse effects on dermatologic diseases. Expand
Functional selectivity of G-protein-coupled receptors: from recombinant systems to native human cells.
  • R. Seifert
  • Chemistry, Medicine
  • Biochemical pharmacology
  • 2013
The current knowledge on functional selectivity of three representative GPCRs, the β2-adrenoceptor and the histamine H2- and H4-receptors, in recombinant systems and native human cells is discussed. Expand
Gene profiling reveals increased expression of uteroglobin and other anti-inflammatory genes in glucocorticoid-treated nasal polyps.
Upregulation of anti-inflammatory genes, such as uteroglobin, might contribute to the effects of local treatment with GCs in nasal polyps. Expand
Cudrania tricuspidata Extract Protects against Reflux Esophagitis by Blocking H2 Histamine Receptors
Findings suggest that C. tricuspidata extracted using ethanol specifically inhibits gastric acid secretion and reduces esophageal injury by blocking H2R in a competitive manner, and may be used in food or medicine to prevent H1R-related diseases. Expand
A discriminating messenger RNA signature for bipolar disorder formed by an aberrant expression of inflammatory genes in monocytes.
The monocytes of a large proportion of bipolar patients and offspring of bipolar parents showed an inflammatory gene expression signature that opens new avenues for biomarker development with possibilities for disease prediction in individuals genetically at risk and for the subclassification ofipolar patients who could possibly benefit from anti-inflammatory treatment. Expand
Inflammatory Monocytes in Bipolar Disorder and Related Endocrine Autoimmune Diseases
With its manic episodes bipolar disorder differs from (unipolar) depression, which is characterized by one or more depressive episodes, but never a manic (or hypomanic) episode. Expand


Stimulation of beta adrenoceptors in a human monocyte cell line (U937) up-regulates cyclic AMP-specific phosphodiesterase activity.
Results indicate that the major PDE isozyme in the soluble fraction of U937 cells is PDE IV and that the activity of this enzyme is increased markedly in cells after prolonged exposure to agents that increase cAMP content. Expand
Regulation of distinct cyclic AMP-specific phosphodiesterase (phosphodiesterase type 4) isozymes in human monocytic cells.
Treatment of peripheral blood monocytes or closely related Mono Mac 6 cells with dibutyryl-cAMP or other cAMP-elevating agents transiently increased rolipram-sensitive PDE4 activity 2-3-fold, without concomitant increases in cGMP-inhibited PDE (PDE3) activity. Expand
Dexamethasone increases intracellular cyclic AMP concentration in murine T lymphocyte cell lines
It is proposed that glucocorticoids interfere with the homeostatic control of intracellular cAMP concentration, leading to a sustained increase in the content of this important second messenger in murine T lymphocyte cell lines. Expand
Anti-inflammatory and immunomodulatory potential of the novel PDE4 inhibitor roflumilast in vitro.
Compared with neutrophils and eosinophils, representing the terminal inflammatory effector cells, the relative potency of roflumilast and its N-oxide for monocytes, CD4+ T cells, and dendritic cells is substantially higher compared with cilomilasts and rolipram, probably reflecting an improved immunomodulatory potential. Expand
Properties and hormonal regulation of two structurally related cAMP phosphodiesterases from the rat Sertoli cell.
Data show that in the rat Sertoli cell, the cAMP-PDE activity is regulated by hormones via a novel mechanism that involves a camp-dependent activation of transcription of a PDE gene. Expand
Elevated leukocyte cyclic AMP-phosphodiesterase in atopic disease: a possible mechanism for cyclic AMP-agonist hyporesponsiveness.
Increased phosphodiesterase activity in mononuclear leukocytes of patients with atopic dermatitis and allergic rhinitis provides an explanation for the blunted cAMP responsiveness in atopic leukocyte responses and may reflect a basic biochemical characteristic relevant to abnormal immunocellular regulation in atopy disease. Expand
Prostaglandin E1 Effects on Adenosine 3′:5′-Cyclic Monophosphate Concentration and Phosphodiesterase Activity in Fibroblasts
Abstract Incubation of L-929 and L-2071 fibroblasts with prostaglandin E1 (PGE1) caused a rapid increase in the cyclic AMP content of these cells. A maximal effect was produced with 0.2 μg PGE1 perExpand
Prostaglandin E 1 effects on adenosine 3':5'-cyclic monophosphate concentration and phosphodiesterase activity in fibroblasts (mouse L cells-tissue culture-enzyme kinetics-prostaglandin homologues).
  • V. Maganiello, M. Vaughan
  • Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1972
It is suggested that the increase in phosphodiesterase activity that appears to be a consequence of prolonged elevation of cyclic AMP concentration may account at least in part for the apparent "refractoriness" to PGE(1) that develops after incubation for several hours with this compound. Expand
The mRNA encoding a high-affinity cAMP phosphodiesterase is regulated by hormones and cAMP.
It is demonstrated that cAMP regulates the expression of one of its own degrading enzymes by an intracellular feedback mechanism that involves changes in mRNA levels, similar to other testicular PDEs. Expand
Cortisol, corticotropin, and β-endorphin responses to corticotropin-releasing hormone in patients with atopic eczema
The blunted response of cortisol and ACTH cannot be explained by hypercortisolism as it is the case in major depression, Rather, it may be related to a prolonged underexposure to hypothalamic CRH or to an increased sensitivity of glucocorticoid feedback inhibition. Expand