Effect of acetylcholine on postjunctional membrane permeability in eel electroplaque

@article{Lassignal1977EffectOA,
  title={Effect of acetylcholine on postjunctional membrane permeability in eel electroplaque},
  author={NANCY L. Lassignal and A. Robert Martin},
  journal={The Journal of General Physiology},
  year={1977},
  volume={70},
  pages={23 - 36}
}
Acetylcholine (ACh) was applied iontophoretically to the innervated face of isolated eel electroplaques while the membrane potential was being recorded intracellularly. At the resting potential (about -85 mV) application of the drug produced depolarizations (ACh potentials) of 20 mV or more which became smaller when the membrane was depolarized and reversed in polarity at about zero membrane potential. The reversal potential shifted in the negative direction when external Na+ was partially… Expand
Analysis of sodium and potassium redistribution during sustained permeability increases at the innervated face of Electrophorus electroplaques
  • H. Lester
  • Medicine, Chemistry
  • The Journal of general physiology
  • 1978
TLDR
S sustained exposure to agonist reduces Na and K concentration gradients and ion redistribution occurs with other drugs, such as batrachotoxin and nystatin, which induce prolonged increases in Na permeability. Expand
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The ionic composition of the currents underlying the acetylcholine (ACh) depolarizations in the identified neurons B1 and B3 of the buccal ganglia of Helix pomatia was analysed and an increase of K+ concentration shifted the ACh equilibrium potential towards more positive potentials. Expand
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  • M. Dekin
  • Chemistry, Medicine
  • The Journal of physiology
  • 1983
TLDR
The results of these experiments indicate that the L‐glutamate activated channel is non‐selective for cations, and the value of the permeability ratios for the physiological cations tested are very similar to those obtained for the acetylcholine activated channel in vertebrate skeletal muscle. Expand
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Permeability to Ca2+ of the acetylcholine receptor channel of denervated mouse muscles in the presence of Na+ and of some other cations
TLDR
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TLDR
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A patch clamp study of the nicotinic acetylcholine receptor of bovine adrenomedullary chromaffin cells in culture.
TLDR
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References

SHOWING 1-10 OF 28 REFERENCES
Reversal of acetylcholine potentials in eel electroplaque.
TLDR
In order to obtain the reversal potential, ACh was applied iontophoretically from a micropipette onto the innervated membrane and the relation between ACh potential amplitude and membrane potential was nonlinear because of a decrease with depolarization of the peak conductance change produced by the drug. Expand
Permeability and internal concentration of ions during depolarization of the electroplax.
  • A. Karlin
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1967
TLDR
Normal depolarizations of both the ininervated and the noninnervated membranes in response to 40 ,gM carbamylcholine on the innervated side are shown, and evidence will be presented indicating that the concentration changes are appreciable and these changes and the permeability changes will be estimated. Expand
Synaptic Electrogenesis in Eel Electroplaques
TLDR
The synaptic components become electrically excitable when the potential across the membrane becomes inside-positive, responding as do the nonsynaptic components, with depolarizing inactivation. Expand
Conductance increases produced by bath application of cholinergic agonists to Electrophorus electroplaques
When solutions containing agonists are applied to the innervated face of an Electrophorus electroplaque, the membrane's conductance increases. The agonist-induced conductance is increased at moreExpand
CORRELATION OF MEMBRANE POTENTIAL AND POTASSIUM FLUX IN THE ELECTROPLAX OF ELECTROPHORUS.
Abstract 1. 1. Exposure of the innervated membrane to high external potassium concentrations or to carbamylcholine results in an initial rapid depolarization with a further gradual decline to aExpand
Ionic properties of the acetylcholine receptor in cultured rat myotubes
TLDR
The acetylcholine reversal potential (Er) of cultured rat myotubes is - 3mV, and under certain circumstances, it is known that the receptor in adult rat and frog muscle can be induced to reversibly shift from behavior described by the equivalent circuit equation to that described byThe Goldman equation. Expand
Ionic permeability changes occurring at excitatory receptor membranes of chemical synapses
TLDR
It is demonstrated that the application of the Goldman–Hodgkin–Katz equation to previous data suggests that an increase in PK as well as PNa probably occurs at most if not all excitatory synapses. Expand
Permeability of the endplate membrane activated by acetylcholine to some organic cations.
TLDR
The present results suggested that the endplate ionic channels can be represented by a pore of about 6.4 A in diameter. Expand
Some properties of conductance changes at the end‐plate membrane during the action of acetylcholine
  • N. Takeuchi
  • Chemistry, Medicine
  • The Journal of physiology
  • 1963
TLDR
The present experiments provide further evidence that the principal conductance change during transmitter action at the end-plate concerns sodium and potassium ions and that chloride ion plays a negligible role. Expand
Voltage dependence of agonist effectiveness at the frog neuromuscular junction: resolution of a paradox.
1. End‐plate currents produced by nerve‐released acetylcholine and iontophoretically applied acetylcholine and carbachol have been recorded from voltage‐clamped frog cutaneous pectoris neuromuscularExpand
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