Effect of Tetramicra brevifilum (Microspora) infection on respiratory-burst responses of turbot (Scophthalmus maximus L.) phagocytes.

@article{Leiro2001EffectOT,
  title={Effect of Tetramicra brevifilum (Microspora) infection on respiratory-burst responses of turbot (Scophthalmus maximus L.) phagocytes.},
  author={Jos{\'e} Manuel Leiro and Raul Benitez Iglesias and Anabel Param{\'a} and Manuel L Sanmart{\'i}n and Florencio M. Ubeira},
  journal={Fish & shellfish immunology},
  year={2001},
  volume={11 7},
  pages={
          639-52
        }
}
  • José Manuel Leiro, Raul Benitez Iglesias, +2 authors Florencio M. Ubeira
  • Published in Fish & shellfish immunology 2001
  • Biology, Medicine
  • In vitro assays were performed to investigate microsporidian-induced intracellular and extracellular production of reactive oxygen species (ROS) by peritoneal-exudate adherent (PEA) cells from turbot. ROS production was quantified using the fluorescent reagents OxyBURST Green H2HFF BSA (extracellular) and OxyBURST Green H2DCFDA succinimidyl ester (intracellular). Five days before assay, the cells had been elicited in vivo by intraperitoneal injection of sodium thioglycollate or spores of… CONTINUE READING

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