Effect of TGF-β1 superfamily members on survival of buffalo (Bubalus bubalis) embryonic stem-like cells.

@article{Sharma2013EffectOT,
  title={Effect of TGF-β1 superfamily members on survival of buffalo (Bubalus bubalis) embryonic stem-like cells.},
  author={Rekha Sharma and Nitin Manchindra Kamble and Aman George and M. P. S. Chauhan and Suresh Kumar Singla and R. S. Manik and Prabhat Palta},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2013},
  volume={48 4},
  pages={569-76}
}
This study examined the effects of supplementation of ES-like cell culture medium with bone morphogenetic protein (BMP)-4 (0, 10, 20 or 100 ng/ml) or Noggin (250, 500 or 750 ng/ml) or TGF-β1 (0, 0.1, 1 or 10 ng/ml) or SB431542 (0, 10, 25 or 50 μm), an inhibitor of TGF-β1 signalling, on survival, colony area and expression level of pluripotency genes in buffalo ES-like cells at passage 40-80, under different culture conditions. BMP-4 supplementation significantly reduced (p < 0.05) colony… CONTINUE READING