Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts.

Abstract

With both chymotrypsin and mutanolysin used to form protoplasts, consistent transformation frequencies of 10 to 10 transformants and transfectants per mug of DNA were achieved. The procedure was used to transform protoplasts of Streptococcus cremoris CS224 at low frequency (5 transformants per mug of DNA). 

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