Effect of PEGylated Magnetic PLGA-PEI Nanoparticles on Primary Hippocampal Neurons: Reduced Nano-neurotoxicity and Enhanced Transfection Efficiency with Magnetofection.

  title={Effect of PEGylated Magnetic PLGA-PEI Nanoparticles on Primary Hippocampal Neurons: Reduced Nano-neurotoxicity and Enhanced Transfection Efficiency with Magnetofection.},
  author={Yanna Cui and Xiao Li and Kristina Zeljic and Shifang Shan and Zilong Qiu and Zhaofang Wang},
  journal={ACS applied materials \& interfaces},
Despite broad application of nanotechnology in neuroscience, the nano-neurotoxicity of magnetic nanoparticles in primary hippocampal neurons remains poorly characterized. In particular, understanding how magnetic nanoparticles perturb neuronal calcium homeostasis is critical when considering magnetic nanoparticles as a non-viral vector for effective gene therapy in neuronal diseases. Here, we address the pressing need to systematically investigate the neurotoxicity of magnetic nanoparticles… 

Rapidly Transducing and Spatially Localized Magnetofection Using Peptide-Mediated Non-Viral Gene Delivery Based on Iron Oxide Nanoparticles

High cell membrane targeting by GET-DNA and MNP co-complexes and magnetic fields allowed further enhancement to endocytotic uptake, meaning that the nucleic acid cargo was rapidly internalized beyond that of GET complexes alone (GET-DNA).

Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape

A variety of ION surface modification alternatives that have emerged for facilitating their endocytic uptake and their timely escape from endosomes are reviewed, with special emphasis on how these can be manipulated for the rational design of cell-penetrating vehicles.

Drug-loaded PEG-PLGA nanoparticles for cancer treatment

  • Dan ZhangLin Liu Minghua Liu
  • Biology, Chemistry
    Frontiers in Pharmacology
  • 2022
An overview of the synthesis and application of PEG-PLGA nanoparticles in cancer treatment is provided and the recent advances in ligand modification for active tumor targeting are discussed.

Nonviral Locally Injected Magnetic Vectors for In Vivo Gene Delivery: A Review of Studies on Magnetofection

The data on the efficiency of the local injections of magnetic nanoparticles that carry genetic information upon application of external magnetic fields was collected and systematized and the perspectives of the development of the method were considered.

Poly(ʟ-Glutamic Acid)-Based Zwitterionic Polymer as Charge Conversional Shielding System for Gene Therapy of Malignant Tumors.

The excellent results are mainly attributed to introducing of the zwitterionic copolymer PAMT, which can shield the positive charges of PEI/shVEGF complex in physiological conditions, while the surface potentials of the shielded complexes change into positive charged in the acid environment.

Stimuli-controllable iron oxide nanoparticle assemblies: Design, manipulation and bio-applications

Magnetic nanocomplexes for gene delivery applications.

In this review, recent research integrating MNPs with a viral or nonviral gene vector is summarized from both technical and application perspectives.

Nanotoxicity in neural regenerative medicine

Improvement of synaptic plasticity by nanoparticles and the related mechanisms: Applications and prospects.



Multimodal analysis of PEI-mediated endocytosis of nanoparticles in neural cells.

Detailed insight is provided into how the intracellular delivery of nanoparticles is mediated by polyethylenimine, which is presently the most commonly used nonviral gene transfer agent, and this fundamental knowledge may also assist in the preparation of next-generationnonviral vectors.

Interaction of spherical silica nanoparticles with neuronal cells: size-dependent toxicity and perturbation of calcium homeostasis.

These findings provide the first evidence that silica nanoparticles can induce toxic effects on neuronal cells in a size-dependent way, and that these effects are related to the degree of perturbation of calcium homeostasis.

Surface functionalized magnetic nanoparticles shift cell behavior with on/off magnetic fields

The surface functionalized MNPs are biocompatible, and demonstrate enhanced intracellular delivery under an applied magnetic field (H), which evinces the targeting ability of MNPs.

Effects of Silver Nanoparticles on Primary Mixed Neural Cell Cultures: Uptake, Oxidative Stress and Acute Calcium Responses

  • A. HaaseS. Rott G. Reiser
  • Biology
    Toxicological sciences : an official journal of the Society of Toxicology
  • 2012
Strong effects of SNP associated with calcium dysregulation and ROS formation in primary neural cells, which were detectable already at moderate dosages, are demonstrated.

Facile synthesis of PEGylated PLGA nanoparticles encapsulating doxorubicin and its in vitro evaluation as potent drug delivery vehicle

A facile synthesis method for PEGylated PLGA nanoparticles encapsulating anti-cancer drug doxorubicin for cancer imaging and therapy provides a simple and robust PLGA-based platform for efficient drug delivery and imaging of cancer cells in vitro and in vivo.