Effect of PCR amplicon size on assessments of clone library microbial diversity and community structure.

Abstract

PCR-based surveys of microbial communities commonly use regions of the small-subunit ribosomal RNA (SSU rRNA) gene to determine taxonomic membership and estimate total diversity. Here we show that the length of the target amplicon has a significant effect on assessments of microbial richness and community membership. Using operational taxonomic unit (OTU… (More)
DOI: 10.1111/j.1462-2920.2008.01857.x

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Cite this paper

@article{Huber2009EffectOP, title={Effect of PCR amplicon size on assessments of clone library microbial diversity and community structure.}, author={Julie A Huber and Hilary G. Morrison and Susan M. Huse and Phillip R. Neal and Mitchell L. Sogin and David B. Mark Welch}, journal={Environmental microbiology}, year={2009}, volume={11 5}, pages={1292-302} }