Effect of Oxidative Stress on the Structure and Function of Human Serum Albumin

  title={Effect of Oxidative Stress on the Structure and Function of Human Serum Albumin},
  author={Makoto Anraku and Keishi Yamasaki and Toru Maruyama and Ulrich Kragh-Hansen and Masaki Otagiri},
  journal={Pharmaceutical Research},
Purpose. Human serum albumin (HSA) was mildly oxidized by a metal–catalyzed oxidation system (MCO–HSA), chloramine–T (CT–HSA) or H2O2 (H2O2–HSA), and the effects of these treatments on the structural, drug–binding and esterase–like properties were studied. Methods. Protein conformation was examined by calorimetric, chromatographic, electrophoretic and spectroscopic techniques. Drug binding was studied by ultrafiltration method, and esterase–like activity was determined using p–nitrophenyl… CONTINUE READING
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