Effect of NaCl on Biofilm Formation of the Isolate from Staphylococcus aureus Outbreak Linked to Ham

@article{Lee2014EffectON,
  title={Effect of NaCl on Biofilm Formation of the Isolate from Staphylococcus aureus Outbreak Linked to Ham},
  author={Soomin Lee and Kyoung-Hee Choi and Yohan Yoon},
  journal={Korean Journal for Food Science of Animal Resources},
  year={2014},
  volume={34},
  pages={257 - 261}
}
The objective of this study was to evaluate the effects of NaCl on the biofilm formations of the isolate from Staphylococcus aureus outbreaks linked to ham. The S. aureus ATCC13565 isolated from ham was exposed to NaCl concentrations of 0%, 2%, 4%, and 6% supplemented in tryptic soy broth (TSB) for 24 h at 35℃, followed by plating 0.1 mL of the culture on tryptic soy agar containing 0%, 2%, 4%, and 6% NaCl, respectively. After incubating at 35℃ for 24 h, the colonies on the plates were… 
View on PubMed
ndsl.kr
17 Citations
Background Citations
5
Results Citations
1

Figures and Tables from this paper

17 Citations

Citation Type

Citation Type

In vitro biofilm formation ability of staphylococci under different growth conditions
TLDR
The study describes models that could provide relevant insights in staphylococcal biofilm formation for food and clinical research and highlighted the need for a careful selection of the assay conditions.
icaA Gene of Staphylococcus aureus Responds to NaCl, Leading to Increased Biofilm Formation.
TLDR
Results indicate that icaA plays an important role in biofilm formation by S. aureus when the pathogen is exposed to NaCl.
Effect of sub-inhibitory concentrations of biocides on the architecture and viability of MRSA biofilms.
Coculture of P. aeruginosa and S. aureus on cell derived matrix - An in vitro model of biofilms in infected wounds.
Combination Susceptibility Testing of Common Antimicrobials in Vitro and the Effects of Sub-MIC of Antimicrobials on Staphylococcus aureus Biofilm Formation
TLDR
The results demonstrate that all antibiotics, except acetylisovaleryltylosin tartrate, effectively reduced the S. aureus biofilm formation and provide the theoretical parameters for the selection of effective antimicrobial combinations in clinical therapy and demonstrate how to correctly use antibiotics at sub-MIC as preventive drugs.
Evaluation of culture conditions for mixed biofilm formation with clinically isolated non-albicans Candida species and Staphylococcus epidermidis on silicone.
Influence of culture conditions for clinically isolated non-albicans Candida biofilm formation.
Amino acid consumption and secretion patterns of Staphylococcus aureus following growth in sub-optimal environmental conditions
TLDR
It was evident that various environmental conditions led to differential profiles of amino acid consumption and secretion, which were significantly reduced in the cells grown in sub-optimal conditions compared with bacteria cells grown under optimal conditions.
The Uptake and Release of Amino Acids by Staphylococcus aureus at Mid-Exponential and Stationary Phases and Their Corresponding Responses to Changes in Temperature, pH and Osmolality
TLDR
It was evident that diverse environmental conditions resulted in differential patterns of amino acid uptake and release during adaptation to designated conditions, including variations in temperature, pH and NaCl.
Environmental factors modulate biofilm formation by Staphylococcus aureus
TLDR
This review addresses the environmental factors and discusses how they affect biofilm formation by S. aureus, one of the major bacterial pathogens that is able to induce both animal and human infections.
...
...

References

SHOWING 1-10 OF 34 REFERENCES
Effect of NaCl on the biofilm formation by foodborne pathogens.
TLDR
The hydrophobicity and auto-aggregation enhanced the biofilm formation during the incubation periods of Listeria monocytogenes, Staphylococcus aureus, Shigella boydii, and Salmonella Typhimurium.
Contribution of culture media and chemical properties of polystyrene tissue culture plates to biofilm development by Staphylococcus aureus.
TLDR
The microtitre plate assay was used to measure the biofilm-forming capability of Staphylococcus aureus isolates and the absorbance of adhered, stained cells was measured at 492 nm using a Multiskan plate reader (Flow Laboratories).
Impact of Food-Related Environmental Factors on the Adherence and Biofilm Formation of Natural Staphylococcus aureus Isolates
TLDR
The results suggested that the prevalence of S. aureus strains on food-processing surfaces is above all conditioned by the ability to adapt to the environmental stress conditions present during food production.
Control of Glucose- and NaCl-Induced Biofilm Formation by rbf in Staphylococcus aureus
TLDR
The results suggest that the rbf gene is involved in the regulation of the multicellular aggregation step of S. aureus biofilm formation in response to glucose and salt and that this regulation may be mediated through the 190-kDa protein.
Different patterns of biofilm formation in Staphylococcus aureus under food-related stress conditions.
Staphylococcal biofilms.
  • M. Otto
  • Biology
    Current topics in microbiology and immunology
  • 2008
TLDR
Novel animal models of staphylococcal biofilm-associated infection have given important information on which factors define biofilm formation in vivo, and constitute an important basis for the development of anti-staphylitiscal drugs and vaccines.
Artificial Pseudomonas aeruginosa biofilms and confocal laser scanning microscopic analysis
  • S. Takenaka, M. Iwaku, E. Hoshino
  • Biology, Medicine
    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy
  • 2001
TLDR
Artificial biofilms of mucoid-type Pseudomonas aeruginosa organisms made by centrifuging the organisms onto the surface of a coverglass and culturing further in broth media supplied continuously may lead to a new quantitative system for estimating the bactericidal efficacy of antibacterial drugs in biofilmms.
Effect of NaCl on Heat Resistance, Antibiotic Susceptibility, and Caco-2 Cell Invasion of Salmonella
TLDR
It is indicated that NaCl in food may cause increased thermal resistance, cell invasion efficiency, and antibiotic resistance of Salmonella.
Mutation of sarA in Staphylococcus aureus Limits Biofilm Formation
TLDR
In every strain other than Newman, including RN6390, simultaneous mutation of sarA and agr resulted in a phenotype like that observed with the sarA mutants, and implementation studies using a sarA clone confirmed that the defect in biofilm formation was due to the SarA mutation.
Genetic regulation of the intercellular adhesion locus in staphylococci
TLDR
How various regulatory factors affect the production of PIA/PNAG in staphylococci is summarized.
...
...