Effect of L‐carnitine on the synthesis of nitric oxide in RAW 264·7 murine macrophage cell line

  title={Effect of L‐carnitine on the synthesis of nitric oxide in RAW 264·7 murine macrophage cell line},
  author={Aslı Koç and Tulin Ozkan and Arzu Zeynep Karabay and Asuman Sunguroglu and Fugen Aktan},
  journal={Cell Biochemistry and Function},
L‐Carnitine (β‐hydroxy‐γ‐trimethyl aminobutyric acid) plays a critical role in inflammatory diseases by modulating inflammatory cell functions. Inducible nitric oxide synthase (iNOS), a proinflammatory enzyme responsible for the generation of nitric oxide (NO), has been implicated in the pathogenesis of inflammatory diseases. Mechanism of action of L‐carnitine on inflammation via iNOS and nuclear factor κB (NF‐κB) is unclear. In this study, we aimed to investigate the effect of L‐carnitine on… 

Human macrophage differentiation induces OCTN2–mediated L‐carnitine transport through stimulation of mTOR–STAT3 axis

SLC22A5/OCTN2 emerges as a novel member of the set of genes markers of macrophage differentiation that is induced during the differentiation of monocytes to macrophages at gene and protein levels.

The effect of L-carnitine on inflammatory mediators: a systematic review and meta-analysis of randomized clinical trials

L-carnitine supplementation was significantly associated with lower levels of CRP in comparison to controls and a slight but statistically significant decrease was observed in IL-6 and TNF-α levels.

Beneficial effect of butyrate, Lactobacillus casei and L-carnitine combination in preference to each in experimental colitis.

The present combination of butyrate, L-carnitine, Lactobacillus casei and the combination of three agents is a suitable mixture in control of experimental colitis and should be trialed in the clinical setting.

Immunohistochemical determination of the extracellular matrix modulation in a rat model of choline‐deprived myocardium: the effects of carnitine

The aim of the study was to investigate the role of MMPs and their inhibitors (TIMPs), in the pathogenesis of choline deficiency‐induced cardiomyopathy, and the way they are affected by carnitine supplementation.

Combined but not single administration of vitamin C and l-carnitine ameliorates cisplatin-induced gastric mucosa damage in male rats.

The study concluded that the combined administration of vitamin C and l-carnitine, but not the single therapy, could prevent the adverse effects of cisplatin on gastric tissue.

The effects of L-carnitine supplementation on indicators of inflammation and oxidative stress: a systematic review and meta-analysis of randomized controlled trials

L-carnitine supplementation was associated with lowering of CRP, IL-6, TNF-α, and MDA, and increasing SOD levels, but did not affect other inflammatory and oxidative stress biomarkers.

The effect of L-carnitine on inflammatory mediators: a systematic review and meta-analysis of randomized clinical trials

The results indicate that L-carnitine reduced inflammatory mediators, especially in studies with a duration of more than 12 weeks, and further studies with different doses and intervention durations are necessary.



Protective effects of mercaptoethylguanidine, a selective inhibitor of inducible nitric oxide synthase, in ligature‐induced periodontitis in the rat

It is proposed that enhanced formation of NO and peroxynitrite plays a significant role in the pathogenesis of periodontitis and that MEG treatment protects against the associated extravasation and bone destruction.

Effects of L‐carnitine treatment on oxidant/antioxidant state and vascular reactivity of streptozotocin‐diabetic rat aorta

It is suggested that the beneficial effects of L‐carnitine treatment partially improve vascular reactivity and antioxidant property beyond its reduction of plasma lipids and it may have an important therapeutic approach in the treatment of diabetic vascular complications.

Regulation of the Expression of Inducible Nitric Oxide Synthase

Abstract Nitric oxide (NO), generated by the inducible isoform of nitric oxide synthase (iNOS), has been described to have beneficial microbicidal, antiviral, antiparasital, immunomodulatory, and

Beneficial effects of L-canavanine, a selective inhibitor of inducible nitric oxide synthase, on lactate metabolism and muscle high energy phosphates during endotoxic shock in rats.

In a rodent model of endotoxinic shock, the continuous infusion of L-canavanine, a selective iNOS inhibitor, improved the systemic hemodynamic parameters and the intracellular bio-energetic patterns estimated by in vivo 31P MRS.

iNOS-mediated nitric oxide production and its regulation.

Selective inhibition of human glial inducible nitric oxide synthase by interferon‐β: Implications for multiple sclerosis

Inhibition of iNOS may provide a mechanism by which IFN‐β can ameliorate inflammation and cytotoxicity in the central nervous system of patients with multiple sclerosis.

Recent advances on the roles of NO in cancer and chronic inflammatory disorders.

Its role in Cancer, the role of NO in myocardial pathophysiology, in central nervous system (CNS) pathologies, and other diseases such as inflammation, asthma, in chronic liver diseases, inflammatory bowel disease (IBD), arthritis, are discussed.

Antioxidant and antiradical activities of L-carnitine.

Comparison of the Effects of l‐Carnitine and Acetyl‐l‐Carnitine on Carnitine Levels, Ambulatory Activity, and Oxidative Stress Biomarkers in the Brain of Old Rats

ALC was effective, unlike l‐carnitine, in decreasing oxidative damage, including MDA, oxo8dG/oxo8G, and nitrotyrosine, at the same dose in old rat brain, and these data suggest that ALC may be a better dietary supplement than l‐ c Carnitine.