Effect of IS900 Gene of Mycobacterium paratuberculosis on Mycobacterium smegmatis

  title={Effect of IS900 Gene of Mycobacterium paratuberculosis on Mycobacterium smegmatis},
  author={Saleh A. Naser and Robert F. Gillespie and Najih A. Naser and Fouad A. K. El-Zaatari},
  journal={Current Microbiology},
Mycobacterium paratuberculosis is mycobactin dependent and contains multiple copies of the IS900 gene that encodes for p43 (46.5K protein). The correlation between the two characteristics has been investigated. A 3.2-kb BamHI fragment from M. paratuberculosis containing the 1.451 kb IS900 gene was cloned in Escherichia coli and Mycobacterium smegmatis with pcDNA II and pNEZ6.3 plasmids, respectively. Surprisingly, the recombinant M. smegmatis grew poorly and slower in 7H9 broth supplemented… CONTINUE READING
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