Effect of Human Erythrocyte Stromata on Complement Activation

  title={Effect of Human Erythrocyte Stromata on Complement Activation},
  author={Roberto Corrocher and Francesco Saverio Tedesco and P Rabusin and Giorgio De Sandre},
  journal={British Journal of Haematology},
Summary. Stroma from either normal or PNH‐likc red cells is capable of inhibiting, to some extent, lysis in the sucrose test and enhancing lysis in the acidified‐serum test. The same opposing effects are displayed by the exclusion peaks from Sephadcx G‐200 obtained from each stroma preparation, suggesting that the same factor could be responsible for both activities. Stromata and peaks also induce lysis of PNH‐likc cells in unacidified serum, indicating activation of complement through the… 

Activation of complement by stroma from normal and paroxysmal nocturnal haemoglobinuria red cells.

Findings may suggest the possible role of diurnal variation of Mg2+ and Ca2+ concentration in precipitating haemolytic attacks and the possibility that small amount of circulating red cell stroma might maintain the haenolysis on PNH RBC.

The Low Ionic Strength Reaction of Human Blood: Relationship between the Binding of Serum Immunoglobulin and Complement to Red Blood Cells and Surface Charge of the Cells

Summary. Using the sucrose haemolysis reaction of Hartmann & Jenkins (1966) as a basic model, the low ionic strength reaction (LISR) of human blood was studied to determine: (1) serum Ig uptake by

Recombinant Human Erythropoietin in the Treatment of Anaemia in Rheumatoid Arthritis

This particular kind of anaemia, anaemia of chronic disease (ACD) is characterised by decreased serum iron and decreased transferrin saturation despite the presence of normal or increased bone marrow iron stores.

Cancer Stem Cell and Gastrointestinal Cancer: Current Status, Targeted Therapy and Future Implications

Findings related to the role and identification of CSCs in GI-cancers are reviewed and the crucial pathways involved in regulatingCSCs populations’ development and drug resistance are discussed, and use of the tumoroid culture to test novel C SCs-targeted cancer therapies are discussed.

Immunohistochemical Expression of Human Epididymis 4 (He4) in Ovarian Serous Carcinoma in Hospital Serdang from the Year 2006- 2013

A retrospective, cross-sectional study of 71 cases histopathologically diagnosed as OSC comprising of 48 cases of HGSC and 23 cases of LGSC to further evaluate the HE4 tissue expression in 2 different grades of OSC in Hospital Serdang.



Mechanisms of immune lysis of red blood cells in vitro. I. Paroxysmal nocturnal hemoglobinuria cells.

Both normal and HANE serum lysed paroxysmal nocturnal hemoglobinuria (PNH) cells when complement was activated by acidification, CoF and inulin, indicating that the terminal steps of complement were activated in the absence of C4, presumably by the alternate or properdin pathway.

Immune Lysis of AET-treated Normal Red Cells (PNH-like Cells)

The very sensitive population of cells and the existence of two populations of cells are distinguishing characteristics of PNH which have not so far been found in health or in any other disease state.

Ham‐positive Red Cells Induced In vitro by N‐Acetylcysteine or D‐Penicillamine

Red cells incubated with N‐acetylcysteine or D‐penicillamine in appropriate concentrations become sensitive to lysis by acidified normal human serum, i.e. become PNH‐like cells and give a positive

C3 shunt activation in human serum chelated with EGTA.

EGTA serum may be a useful and simple reagent to distinguish between C142 and C3 shunt-mediated complement activation.

On the Mechanism of the In Vitro Production of Ham‐positive Red Cells by Sulphydryl Compounds: Role of pH and Free ‐SH Groups

Experiments were carried out in order to obtain high percentages of Ham‐positive red cells (HPRC) from normal human red cells incubated with various ‐SH containing compounds and to evaluate the role

Diagnostic specificity of sucrose hemolysis test for paroxysmal nocturnal hemoglobinuria.

Because of the clear, colorless character of serum-sucrose mixtures, an insignificant degree of hemolysis is more readily visible than in other PNH hemolytic tests employing undiluted serum.


In the paper by G. Sirchia, S. Ferrone and F. Mercuriali: "The Action of Two Sulfhydryl Compounds on Normal Human Red Cells. Relationship to Red Cells of Paroxysmal Nocturnal Hemoglobinuria,"

Paroxysmal Nocturnal Hemoglobinuria

Findings at MR imaging and CT were the results of a deposition of hemosiderin in the cells of proximal convoluted tubules, and were helpful for diagnosis of PNH.