C 1 2 3 DNA size (kb) log C of ethidium bromide ( μ g/ml × 100) Fig. 2. Summarized results of nuclease DNA digestion in acetylated nuclei after ethidium bromide titration
- J. Allan, N. Harborne, D. C. Rau, H. Gould
- J. Cell Biol
According to theoretical estimations, the tertiary conformation of a nucleosome fiber depends on parameters of the nucleosome–nucleosome linker system, including the rotation angle between adjacent nucleosomes. We studied the effect of histones with modified primary structure on chromatin-fiber folding; ethidium bromide was used to measure the internucleosome DNA axial twist. It was found that hyperacetylation of histone terminal domains and their selective enzymatic removal led to specific structural transitions in chromatin fibers because of changes in the linker DNA twist.